Identification and Expression of Some Plant Cell Wall-Degrading Enzymes Present in Three Ontogenetics Stages of Thecaphora frezii, a Peanut (<i>Arachis hypogaea</i> L.) Pathogenic Fungus

Peanuts can be affected by the presence of pathogenic microorganisms. The fungus Thecaphora frezii (T. frezii), which belongs to taxonomic class Ustilaginomycetes, is causal agent disease known as “peanut smut”. life cycle this includes three stages, namely teliospores, basidiospores and hyphae. In hyphae stage, infection occurs in peanut plant, requires involvement some enzymes secreted fungus. These include Plant Cell Wall-Degrading Enzymes (PCWDEs), degrade various polysaccharides. This study aimed identify transcript for belonging PCWDEs from stages T. frezii. For this, total RNA was extracted ontogenetic samples were analyzed using an RNA-Seq approach transcripts quantified Real Time PCR. analysis data provided it possible that could encode compatible with polysaccharides degradation are part plant cell wall. transcriptome, 40 deduced proteins would functions identified. They divided into 27 glycoside hydrolases; two polysaccharide lyases; carbohydrate esterases eight auxiliary activities. addition, fungal SNF1 gene identified whose activity high glucose level, indirectly influence levels PCWDEs. help understand process possibly find substances control its development.